Recombinant Murine GM-CSF Protein
Catalog Number: TL-655
Gene Name Synonym: CSF-2, mgi-1gm, pluripoietin-α
Construction: DNA sequences encoding murine GM-CSF (NP_034099.2) were expressed with His tag at C terminal.
Source::Murine
Expression Host: HEK293
QC Testing Purity::> 95 % as determined by SDS-PAGE
Bio Activity: ED50 dose dependent stimulation of mouse FDC-P1 cell proliferation was ≤0.05ng/ml, corresponding specific activity ≥ 2×107IU/mg.
Endotoxin: < 0.1EU per ug of the protein as determined by the LAL method.
Molecular Mass: The recombinant Murine GM-CSF contains 130 amino acids, with a predicted theoretical molecular weight of 15kd.
Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % mannitol are added as protectants before lyophilization.
Stability & Storage: Storage& Stability Samples are stable for up to 24 months from date of receipt at 4 ℃.
Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Protein Description
GM-CSF is a hematopoietic growth factor that promotes the development of neutrophils and macrophages, as well as early erythroid megalogues and eosinophilic progenitors cell proliferation and development. It is produced by endothelial cells, monocytes, fibroblasts and T lymphocytes. GM-CSF inhibits the migration of neutrophils shift and enhance the functional activity of mature terminal cells. The human and mouse molecules are species-specific and do not exhibit cross-species reactivity.
References
1. Relative Efficacy of Granulocyte-Macrophage Colony-Stimulating Factor, Dacarbazine, and Glycoprotein 100 in Metastatic Melanoma: An Indirect Treatment Comparison. Quinn C, Ma Q, Kudlac A, Palmer S, Barber B, Zhao Z. Adv Ther. 2017;34(2):495-512.
2. Dual Role of GM-CSF as a Pro-Inflammatory and a Regulatory Cytokine: Implications for Immune Therapy. Bhattacharya P, Budnick I, Singh M, Thiruppathi M, Alharshawi K, Elshabrawy H, et al. J Interferon Cytokine Res. 2015;35(8):585-99.
3. GM-CSF: An immune modulatory cytokine that can suppress autoimmunity. Bhattacharya P, Thiruppathi M, Elshabrawy HA, Alharshawi K, Kumar P, Prabhakar BS. Cytokine. 2015;75(2):261-71.
Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).
