Recombinant Human IL-15 Protein
Catalog Number:TL-202
Product name
Generic names: Recombinant Human IL-15 Protein
Gene Name Synonym: IL-15, Interleukin 15 Protein
Product information
Construction: A DNA sequence encoding the extracellular domain of human IL-15 (NP_000576.1) was expressed with the C-terminal fused Fc region of human IgG1.
Source: Human
Expression Host: CHO cells
QC Testing Purity: > 95 % as determined by SDS-PAGE
Bio Activity: The ED 50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 cellswas found to be ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2×10 6 units/mg..
Endotoxin: < 0.01EU per mg of the protein as determined by the LAL method.
Molecular Mass: The recombinant human IL-15 consists of 362 amino acids and predicts a molecular mass of 45 kDa.
Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.
Stability & Storage: Samples are stable for up to 24 months from date of receipt at 4 ℃ . Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Background
The protein encoded by IL-15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This
cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor
subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of
CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL2. IL-15 induces the activation of JAK
kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of
the mouse counterpart suggested that IL-15 may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L),
possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced
transcript variants of this gene have been reported.
References
1.Mongini PKA, Gupta R, Boyle E, et al. TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic
lymphocytic leukemia B cells. Journal of immunology (Baltimore, Md?: 1950). 2015;195(3):901-923.
Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).
