Recombinant Human Fibronectin Fragment
Catalog Number: TL-103
Gene Name Synonym: FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND
Construction: Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed with a 6His tag at the N-terminus.
Source: Human
Expression Host: Escherichia coli.
AASequence: HHHHHHPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSMAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST
QC Testing Purity: > 95 % as determined by SDS-PAGE
Bio Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 1×105 cells/well areadded to Fibronectin-coated plates (7-13 ng/mL and 100μL/well), approximately 50%-80% will adhere specifically after 30 minutes at 37°C.
Molecular Mass: The recombinant human Fibronectin consists of 580 amino acids and predicts a molecular mass of 63.5KDa.
Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.
Stability & Storage: Samples are stable for up to 24 months from date of receipt at 2~8 ℃.
Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Protein Description
Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
References
1.Williams CM, Engler AJ, Slone RD, Galante LL, Schwarzbauer JE (2008). Fibronectin expression modulates mammary epithelial cell proliferation during acinar differentiation. Cancer Research 68 (9): 3185–92.
2.Mao Y, Schwarzbauer JE (2005). Fibronectin fibrillogenesis, a cell-mediated matrix assembly process. Matrix Biology : 3. Journal of the International Society for Matrix Biology 24 (6): 389–99.
Erickson HP (2002) .Stretching fibronectin. Journal of Muscle Research and Cell Motility 23 (5-6): 575–80.
Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).
