Product Description

Recombinant Human IL-21 Protein
Catalog Number: TL-509

Product name
Generic names: Recombinant Human IL-21 Protein
Gene Name Synonym: CVID11, IL-21, Za11

Product information
Construction: A DNA sequence encoding the extracellular domain of human IL-21 (Q9HBE4-1)) was expressed with the C-terminal fused Fc region of human IgG1.
Source: Human
ExpressionHost:  CHO cells
QC Testing Purity: > 95 % as determined by SDS-PAGE
Bio Activity: Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED 50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x10^6units/mg.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Molecular Mass: The recombinant human IL-21 consists of 366 amino acids and predicts a molecular mass of 41 Kda.
Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.
Stability & Storage: Samples are stable for up to 24 months from date of receipt at 4 ℃ . Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Background
IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally
enhances antigen-specific responses of immune cells. The biological effects of IL-21 include: inducing the
differentiation of T-cell-stimulated B-cells into plasma cells and memory B-cells; the stimulation of IgG production in
conjunction with IL-4; and the induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of
T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its
effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other
cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events,
which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors
STAT1 and STAT3.

References
1. Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production.
BEIJING T&L BIOLOGICAL TECHNOLOGY CO.,LTD. For research use only.J Immunol. 178(5):2827-34.
2. Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J
Biol Chem. 282(48):34605-10. G&W Biotechnologie GmbH For research use only.
3. Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J
Leukoc Biol. 72(5):856-63.
4 .Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during
CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).

Payment Terms︰ TT