Product Description

Recombinant Human IL-7 Protein

Catalog Number: TL-506

 

Product name

Generic names:   Recombinant Human IL-7 Protein

Gene Name Synonym:   Lymphopoietin 1(LP-1), pre-B cell factor

 

Product information

Construction:  A DNA sequence encoding the extracellular domain of human IL-7 (NP_000871.1) was expressed with the C-terminal fused Fc region of human IgG1.

Source:   Human

Expression Host:  CHO cells

QC Testing Purity:   > 95 % as determined by SDS-PAGE

Bio Activity:  The ED 50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2×10 6 units/mg.

Endotoxin:   < 0.01EU per mg of the protein as determined by the LAL method.

Molecular Mass:  The recombinant human IL-7 consists of 395 amino acids and predicts a molecular mass of 42.5 KDa.

Formulation:  Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.

Stability & Storage:  Samples are stable for up to 24 months from date of receipt at 4 ℃ . Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

 

Background

IL-7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted

by stromal cells in the bone marrow and thymus. IL-7 stimulates the proliferation of lymphoid progenitors. Produced by

thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in

combination with other factors, such as ConA and IL-2. It is important for proliferation during certain stages of B-cell

maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell

growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during

early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve

as a regulatory factor for intestinal mucosal lymphocytes. IL-7 is a hematopoietic growth factor that primarily affects

early B and T cells.

 

References

1. Watanabe M, et al. (1995) Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation

BEIJING T&L BIOLOGICAL TECHNOLOGY CO.,LTD. For research use only.of intestinal mucosal lymphocytes.

2. J Clin Invest. 95(6):2945-53. Sawa Y, et al. (2009) Hepatic interleukin-7 expression regulates T cell responses.

Immunity. 30 (3):447- 57.

3. Flad HD, et al. (1996) Human follicular dendritic cells and vascular cells produce interleukin-7:  a potential role for

interleukin-7 in the germinal center reaction. Eur J Immunol. 26(10):  2541-4.

 

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).

Payment Terms︰ TT