Product Description

Recombinant Human PDGF-BB Protein

Catalog Number: TL-644

Gene Name Synonym: PDGFBB(Platelet-Derived Growth Factor-BB),  PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF).

Construction: A DNA sequence encoding the human PDGF-BB (GenBank: CAA45383.1) was expressed with a polyhistidine tag at the C-terminus.

Source::Human

Expression Host: HEK293 cells

QC Testing Purity: > 95 % as determined by SDS-PAGE

Bio Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.The expected ED 50 for this effect is 1.0-3.0 ng/ml.

Endotoxin: <0.01 EU per μg of the protein as determined by the LAL method.

Molecular Mass: The Recombinant Human PDGF-BB consists of 115 amino acids and predicts a molecular mass of 13.1 KDa.

Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization.

Stability & Storage: Samples are stable for up to 24 months from date of receipt at 4 ℃.

Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Protein Description

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB.

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).

Payment Terms︰ TT