Product Description

Humanized Anti-Human CD16 mAb

Catalog Number: TL-201

Product name

Generic names:  Humanized Anti-Human CD16 mAb

Product information

Species Reactivity:  Human

Specificity:  Detects mouse Fcγ RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no crossreactivity with recombinant mouse Fcrl3/CD162, rmFcγ RIA or rhFcγ RIIA is observed

Source:  CHO cells

QC Testing Purity:  > 95 % as determined by SDS-PAGE

Purification:  Protein A purified from cell culture supernatant

Immunogen:  Recombinant human CD16A

Endotoxin Level:  <0.1 EU/μg

Formulation:  Lyophilized from a 0.2 μm filtered solution in PBS with HSA

Stability & Storage: Samples are stable for up to 24 months from date of receipt at 4 ℃.Avoid repeated freeze-thaw cycles.

Applications: 1.ELISA(recommended concentration 1:2000-1:5000) 2. T Cell Stimulation: This antibody can be used to activate T cells when immobilized at 1-10

μg/mL (100 μL/well)

Background

Receptors for the Fc region of IgG (Fc γ Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity),cytokine release, and B cell proliferation (1-3). The Fc γ Rs have been divided into three classes based on close relationships in their extracellular domains these groups are designated Fc γ RI (also known as CD64), Fc γ RII (CD32),and Fc γ RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10 -8 -10 -9 M) capable of binding monomeric IgG,whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10 -6 -10 -7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc γ Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc Rγ or ζ (3, 5). The only inhibitory member in human and mouse, Fc γ RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc Rγ to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95%BEIJING T&L BIOLOGICAL TECHNOLOGY CO.,LTD. For research use only.

amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.

2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.

3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.

4. Takai, T. (2002) Nature Rev. Immunol. 2:580.

5. Ravetch, J. and L. Lanier (2000) Science 290:84.

 

Trial dosage: A customer can apply for up to five samples a year (the actual trial dosage is determined by telephone or email communication).
Scope of use: Samples are limited to testing and research purposes.
Cost: Free for samples, customer is responsible for shipping and handling charges, reference cost is $50 in the US, €60 in the EU, and $50 in other countries.
How to apply: You can apply by telephone or by email (the email application should specify the sample name, item number, contact person, telephone number, consignee address, etc.).

Payment Terms︰ TT